GlycoSolutions

Elizabeth Higgins, Ph.D. return to home page

EDUCATION:

MBA, Boston University, Boston, Massachusetts, U.S.A.

Ph.D. University of Toronto, Toronto, Ontario, Canada

B.Sc. (Honours), University of Guelph, Guelph, Ontario, Canada

PROFESSIONAL ORGANIZATIONS:

2000-present ABRF (Association of Biomolecular Resource Facilities)

Carbohydrate Analysis Research Group

1994-2001 Board of Directors, Boston Glycobiology Discussion Group

EXPERIENCE:

Group Leader, Glycobiology Group, Genzyme Corporation.

Develop assays for carbohydrate analysis

Characterization of the glycosylation of recombinant and transgenic proteins

Author of sections of submissions to the FDA and international regulatory agencies

Remodeling of oligosaccharide structures on glycoproteins

Protein characterization (including protein degradation pathways and product consistency/comparability studies)

Postdoctoral Fellow, Genzyme Corporation and the Massachusetts Institute of Technology.

Developed a technique for analyzing the glycosylation of recombinant proteins from conditioned medium (without purification) using FACE analysis.

Studied the effect of environmental factors on the glycosylation of recombinant proteins.

PRESENTATIONS:

1. Glyco 2000 (November, 2000) "The Analysis of Mannose-6-Phosphorylated Oligosaccharides on Lysosomal Enzymes"

2. Glyco XV (August, 1999) "Thryogen^Ò : New Glycosylation on an Old Glycoprotein"

3. Boston Glycobiology Discussion Group (October, 1998) "The Glycosylation of Recombinant Glycoproteins Expressed in the Milk of Transgenic Goats"

4. ABRF (May 1998) "Fluorophore Assisted Carbohydrate Electrophoresis (FACE) as a Quantitative Oligosaccharide Profiling Method"

5. Third International Glycobiology Symposium (November, 1995) "A Comparison of Oligosaccharide Profiling Methods"

6. XVII^th International Carbohydrate Symposium (July, 1994) "Decreasing the Serum Clearance Rate of Human Antithrombin III, Expressed in the Milk of Transgenic Goats, by Enzymatic Modification of its N-glycosylation"

7. American Association for the Advancement of Science (AAAS). Science Innovation. (August, 1993) "Routine Analysis of Recombinant Protein Glycosylation Patterns, without Protein Purification, by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE).

RECENT ABSTRACTS:

1. The Stuctural Characterization of N-linked Oligosaccharides from Recombinant Human Antithrombin III using HPAEC-PAD and MALDI-TOF MS. Kyazike, J., Zhou, Q and E. Higgins (2001) 6^th Annual Conference of the Society for Glycobiology.

2. Comparison of Oligosaccharide Profiling Methods for Recombinant Glycoproteins Intended for Therapeutic Use: High pH Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) Versus Fluorescent Labeling (2-anthranilic acid). Granda, B., Higgins E., Théberge, R., Mattaliano, R. and S. Ganesa (2001) 6^th Annual Conference of the Society for Glycobiology.

PUBLICATIONS:

1. Zhou, Q., Kyazike, J., Edmunds, T. and E. Higgins (2002) Mannose 6-Phosphate Quantitation in Glycoproteins Using High-pH Anion Exchange Chromatography with Pulsed Amperometric Detection. Anal. Biochem. 306: 163-170

2. O’Riordan, C.R., Lachapelle, A.L., Marshall, J., Higgins, E.A., and S.H. Cheng (2000) Characterization of the oligosaccharide structures associated with the cystic fibrosis transmembrane conductance regulator. Glycobiology 10:1225-1234

3. Edmunds, T., van Patten, S.M., Pollock, J., Hanson, E., Bernasconi, R., Higgins, E., Manavalan, P., Ziomek, C., Meade, H., McPherson, J.M., and E.S. Cole (1998) Transgenically produced human antithrombin: structural and functional comparison to human plasma-derived antithrombin. Blood 91:4561-71

4. Higgins, E., and R. Bernasconi (1996) A comparison of oligosaccharide profiling methods. Techniques in Glycobiology Vol I

5. Price, A.E., Logvinenko, K.B., Higgins, E.A., Cole, E.S., and S.M. Richards (1995) Studies on the microheterogeneity and in vitro activity of glycosylated and nonglycosylated recombinant human PRL separated using a novel purification process. Endocrinol. 136:4827-4833

6. Friedman, Y., and E. Higgins (1995) A method for monitoring the glycosylation of recombinant glycoproteins from conditioned medium using polyacrylamide gel separation of fluorescently labelled oligosaccharides. Anal. Biochem. 228:221-225

7. Simon, H.-U., Higgins, E.A., Demetriou, M., Datti, A., Siminovitch, K.A., and J.W. Dennis (1993) Defective expression of CD23 and autocrine growth-stimulation in Epstein-Barr virus (EBV)-transformed B cells from patients with Wiskott-Aldrich syndrome (WAS). Clin. Exp. Immunol. 91:43-49

8. Zhuang, D., Grey, A., Harris-Brandts, M., Higgins, E., Kashem, M., and J. Dennis (1991) Characterizaton of O-linked oligosaccharide biosynthesis in cultured cells using paranitrophenyl a -D-GalNAc as an acceptor. Glycobiology 1:425-433.

9. Higgins, E., Siminovitch, K., Zhuang, D., Brockhausen, I., and J. Dennis (1991) Aberrant O-linked oligosaccharide biosynthesis in lymphocytes and platelets from patients with the Wiskott-Aldrich syndrome. J. Biol. Chem. 266:6280-6290.

10. Yousefi, S., Higgins, E., Zhuang, D., Hindsgaul, O., and J . Dennis(1991) Increased UDP-GlcNAc:Galb1-3GalNAc-R (GlcNAc to GalNAc) b1-6 N-acetylglucosaminyltransferase activity in metastatic murine tumor cell lines: Control of polylactosamine synthesis. J. Biol. Chem. 266:1772-1782.

11. Greer, W., Higgins, E., Sutherland, D., Novogrodsky, A., Brockhausen, I., Peacocke, M., Rubin, L., Baker, M., Dennis, J., and K. Siminovitch. (1989) Altered expression of leucocyte sialoglycoprotein in Wiskott-Aldrich syndrome is associated with a specific defect in O-glycosylation. Biochem. Cell Biol. 67:503-509.

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