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Peptide Mapping

In our tryptic peptide analysis, samples are reduced and alkylated then digested with trypsin. The resulting tryptic peptide mixture is analyzed by reversed-phase HPLC with UV detection.

The tryptic peptide mapping analysis provides a qualitative profile or map of the peptides generated from a trypsin digest of a sample.

GlycoPeptide Mapping

In our GlycoPeptide analysis, samples that have been deglycosylated using the enzyme PNGase F are reduced, alkylated and subsequently digested with trypsin. An additional sample is required for analysis without deglycosylation.  The resulting tryptic peptide mixtures are analyzed by reversed-phase HPLC with UV detection.  Glycosylated peptides can be identified as peaks that disappear/appear in the chromatograms.

The GlycoPeptide Mapping Analysis provides a qualitative profile or map of the peptides generated from digests of glycosylated and deglycosylated samples. Comparison of the peptide maps aids in identifying the glycopeptides in the peptide map and in determining the number of occupied N-glycosylation sites.

Reversed Phase Chromatography

Size Exclusion Chromatography

Sample Submission Form

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